Explain tailing factor and how it affects quantitation and resolution. What causes tailing?

Explain tailing factor and how it affects quantitation and resolution. What causes tailing?

• A. Tailing factor > 1 indicates asymmetrical peak; causes include silanol interactions, overloading, solvent mismatch, column degradation, buffer issues.
• B. Tailing improves resolution.
• C. Tailings are caused by detector noise.
• D. Tailing factor near 1 indicates severe tailing.

Answer: (A)